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Core Facilities
Genotyping
Members
Overview
Genotyping on a medium to high-throughput scale is a powerful tool in plant breeding. Our service offers marker (mainly SNPs and SSRs or microsatellites) development and application for public research projects as well as private breeding programs within a broad spectrum of plant species.
Equipment
ABI PRISM 3130xl Genetic Analyzer
Separation of DNA fragments by capillary electrophoresis
Applications
Custom Made Plant Genotyping
CULTIVAR IDENTIFICATION
- Germplasm characterization
- Genetic variability studies
- Pedigree and quality control
HYBRID PURITY TESTING
- Purity test of hybrid seed production
- New hybrid and pedigree control
MAJOR GENE DETECTION
- Genetic map construction
- QTL analysis
- Association genetics
MARKER-ASSISTED SELECTION (MAS)
- In a wide range of species: resistance genes
- Prunus: acidity, round/flat fruit shape (paraguayo or chato), fruit flesh colour and texture, peach/nectarine, flowering time and maturity date, auto-compatibility and male sterility
SPECIAL BREEDING APPLICATIONS
Whole genome selection (e.g. introduction of exotic alleles)