The group intends the control of plant pathogenic viruses through interference with the transmission processes, and through the use of genetic resistance.
For the first part, we are focusing recently in mixed infections caused by unrelated plant viruses. Different pathosystems are under study, such as combinations in susceptible plants of different aphid-transmitted potyviruses with whitefly-transmitted criniviruses, and also including other whitefly-transmitted ipomoviruses and begomoviruses. Crop plants chosen belong to the cucurbitaceae, solanaceae and convolvulaceae families. We are considering the dynamics of the interactions between the different viruses, and the effects caused by the multiple infections in the dissemination by insect vectors. In addition, we want to analyze the modulation of the activity of viral suppressors of RNA silencing.
The objectives on genetic resistance are mainly focused on Cucumber Mosaic virus (CMV) in melon. We aim to genetically unveil the complex resistance to CMV in melon composed of at least one gene (cmv1) and two QTLs. We are interested in understanding the molecular mechanism by which cmv1, encoding a Vacuolar Protein Sorting 41, is able to stop the viral transport to the phloem, restricting the virus to the bundle sheath cells and preventing a systemic infection. For that we are using a collection of melon germplasm and a collection of CMV strains. To fully understand the complex resistance to CMV in melon, we also aim to identify the other two QTLs controlling the resistance to the aggressive CMV strains. We have also set up CRISPR/Cas9 in melon as a tool for validation of candidate genes.
Giordano A, Ferriol I, Lopez-Moya JJ and Martin Hernandez AM
cmv-1 mediated resistance to CMV in melon can be overcome by mixed infections with potyviruses
(2023) Viruses 15(9):1792 ,DOI: 10.3390/v15091792
Chase O, Javed A, Byrne MJ, Thuenemann EC, Lomonossoff GP, Ranson NA and Lopez-Moya JJ
CryoEM and stability analysis of virus-like particles of potyvirus and ipomovirus infecting a common host
(2023) Communications Biology 6(1):443, DOI: 10.1038/s42003-023-04799-x
Real N.; Villar I.; Serrano I.; Guiu-Aragonés C.; Martín-Hernández A.M
Mutations in CmVPS41 controlling resistance to Cucumber mosaic virus display specific subcellular localization.
(2023) Plant Physiology, vol. 191 (3), pp. 1596 -1611 (DOI:https://doi.org/10.1093/plphys/kiac583)
Giordano A., Santo Domingo M., Quadrana L., Pujol M., Martín-Hernández A.M., Garcia-Mas J.
CRISPR/Cas9 gene editing uncovers the roles of CONSTITUTIVE TRIPLE RESPONSE 1 and REPRESSOR OF SILENCING 1 in melon fruit ripening and epigenetic regulation
(2022) Journal of Experimental Botany, vol. 73 (12), pp. 4022 -4033 (DOI:10.1093/jxb/erac148)
Liu B., Santo Domingo M., Mayobre C., Martín-Hernández A.M., Pujol M., Garcia-Mas J.
Knock-Out of CmNAC-NOR Affects Melon Climacteric Fruit Ripening
(2022) Frontiers in Plant Science, vol. 13 Art. number 878037 (DOI:10.3389/fpls.2022.878037)
Ontiveros I., López-Moya J.J., Díaz-Pendón J.A.
Coinfection of Tomato Plants with Tomato yellow leaf curl virus and Tomato chlorosis virus Affects the Interaction with Host and Whiteflies
(2022) Phytopathology, vol. 112 (4), pp. 944 -952 (DOI:10.1094/PHYTO-08-21-0341-R)
Desbiez C., Domingo-Calap M.L., Pitrat M., Wipf-Scheibel C., Girardot G., Ferriol I., Lopez-Moya J.J., Lecoq H.
Specificity of Resistance and Tolerance to Cucumber Vein Yellowing Virus in Melon Accessions and Resistance Breaking with a Single Mutation in VPg
(2022) Phytopathology, vol. 112 (5), pp. 1185 -1191 (DOI:10.1094/PHYTO-06-21-0263-R)
Thuenemann E.C., Byrne M.J., Peyret H., Saunders K., Castells-Graells R., Ferriol I., Santoni M., Steele J.F.C., Ranson N.A., Avesani L., Lopez-Moya J.J., Lomonossoff G.P.
A replicating viral vector greatly enhances accumulation of helical virus-like particles in plants
(2021) Viruses, vol. 13 (5), Art. number 885 (DOI:10.3390/v13050885)
Domingo-Calap M.L., Chase O., Estapé M., Moreno A.B., López-Moya J.J.
The P1 Protein of Watermelon mosaic virus Compromises the Activity as RNA Silencing Suppressor of the P25 Protein of Cucurbit yellow stunting disorder virus
(2021) Frontiers in Microbiology, vol. 12 Art. number 645530 (DOI:10.3389/fmicb.2021.645530)
Martín-Hernández A.M., Picó B.
Natural resistances to viruses in cucurbits
(2021) Agronomy, vol. 11 (1), Art. number 23 (DOI:10.3390/agronomy11010023)
Domingo-Calap M.L., Moreno A.B., Pendón J.A.D., Moreno A., Fereres A., López-Moya J.J.
Assessing the impact on virus transmission and insect vector behavior of a viral mixed infection in melon
(2020) Phytopathology, vol. 110 (1), pp. 174 -186 (DOI:10.1094/PHYTO-04-19-0126-FI)
Moreno A.B., López-Moya J.J.
When viruses play team sports: Mixed infections in plants
(2020) Phytopathology, vol. 110 (1), pp. 29 -48 (DOI:10.1094/PHYTO-07-19-0250-FI)