The group intends the control of plant pathogenic viruses through interference with the transmission processes, and through the use of genetic resistance.
For the first part, we are focusing recently in mixed infections caused by unrelated plant viruses. Different pathosystems are under study, such as combinations in susceptible plants of different aphid-transmitted potyviruses with whitefly-transmitted criniviruses, and also including other whitefly-transmitted ipomoviruses and begomoviruses. Crop plants chosen belong to the cucurbitaceae, solanaceae and convolvulaceae families. We are considering the dynamics of the interactions between the different viruses, and the effects caused by the multiple infections in the dissemination by insect vectors. In addition, we want to analyze the modulation of the activity of viral suppressors of RNA silencing.
The objectives on genetic resistance are mainly focused on Cucumber Mosaic virus (CMV) in melon. We aim to genetically unveil the complex resistance to CMV in melon composed of at least one gene (cmv1) and two QTLs. We are interested in understanding the molecular mechanism by which cmv1, encoding a Vacuolar Protein Sorting 41, is able to stop the viral transport to the phloem, restricting the virus to the bundle sheath cells and preventing a systemic infection. For that we are using a collection of melon germplasm and a collection of CMV strains. To fully understand the complex resistance to CMV in melon, we also aim to identify the other two QTLs controlling the resistance to the aggressive CMV strains. We have also set up CRISPR/Cas9 in melon as a tool for validation of candidate genes.
Moreno A.B., López-Moya J.J.
When viruses play team sports: Mixed infections in plants
(2020) Phytopathology, vol. 110 (1), pp. 29 -48 (DOI:10.1094/PHYTO-07-19-0250-FI)
Domingo-Calap M.L., Moreno A.B., Pendón J.A.D., Moreno A., Fereres A., López-Moya J.J.
Assessing the impact on virus transmission and insect vector behavior of a viral mixed infection in melon
(2020) Phytopathology, vol. 110 (1), pp. 174 -186 (DOI:10.1094/PHYTO-04-19-0126-FI)
Pascual L., Yan J., Pujol M., Monforte A.J., Picó B., Martín-Hernández A.M.
CmVPS41 Is a General Gatekeeper for Resistance to Cucumber Mosaic Virus Phloem Entry in Melon
(2019) Frontiers in Plant Science, vol. 10 Art. number 1219(DOI:10.3389/fpls.2019.01219)
Rodamilans B., Valli A., Mingot A., San León D., López-Moya J.J., García J.A.
An atypical RNA silencing suppression strategy provides a snapshot of the evolution of sweet potato-infecting potyviruses.
(2018) Scientific Reports, 8 (1): 15937
Valli A.A., Gallo A., Rodamilans B., López-Moya J.J., García J.A.
The HCPro from the Potyviridae family: an enviable multitasking Helper Component that every virus would like to have.
(2018) Molecular Plant Pathology, 19 (3): 744-763
Cordero T., Mohamed M.A., López-Moya J.-J., Daròs J.-A.
A recombinant Potato virus Y infectious clone tagged with the Rosea1 visual marker (PVY-Ros1) facilitates the analysis of viral infectivity and allows the production of large amounts of anthocyanins in plants.
(2017) Frontiers in Microbiology, 8: 611
J. Argyris, A. Díaz , V. Ruggieri, M. Fernández, T. Jahrmann, Y. Gibon, B. Picó, A. M. Martín-Hernández, A.J. Monforte and J. Garcia-Mas.
QTL analyses in multiple populations employed for the fine mapping and identification of candidate genes at a locus affecting sugar accumulation in melon (Cucumis melo L.).
(2017) Frontiers in Plant Science, 8: 1679.
A. Giner, L. Pascual, M. Bourgeois, G. Gyetvai, P. Rios, B. Picó, C. Troadec, A. Bendahmane, J. Garcia-Mas and A. M. Martín-Hernández.
A mutation in the melon Vacuolar Protein Sorting 41 prevents systemic infection of Cucumber mosaic virus.
(2017) Scientific Reports, 7: 10471
A. Díaz, A. M. Martin-Hernandez, R. Dolcet-Sanjuan, A. Garces-Claver1, J. M. Álvarez, J. Garcia-Mas, B. Picó and A. J. Monforte
Quantitative trait loci analysis of melon (Cucumis melo L.) domestication-related traits.
(2017)Theoretical and Applied Genetics,130 (9):1837-1856.
C. Guiu-Aragonés, M. A. Sánchez-Pina, J. A. Díaz-Pendón, E. J. Peña, M. Heinleinand A. M. Martín-Hernández.
cmv1 is a gate for Cucumber mosaic virus transport from bundle sheath cells to phloem in melon.
(2016) Mol. Plant Pathology, 17: 973-984.
Mingot A., Valli A., Rodamilans B., León D.S., Baulcombe D.C., García J.A., López-Moya J.J.
The P1N-PISPO trans-frame gene of sweet potato feathery mottle potyvirus is produced during virus infection and functions as an RNA silencing suppressor.
(2016) Journal of Virology, 90(7): 3543-3557
Guiu-Aragonés C., Díaz-Pendón J.A., Martín-Hernández A.M.
Four sequence positions of the movement protein of Cucumber mosaic virus determine the virulence against cmv1-mediated resistance in melon.
(2015) Molecular Plant Pathology, 16 (7): 675-684