Group Leaders
Nicolas Bologna
Junior Leader Incoming Fellowship la Caixa
Group Members
RNA biology group


The purpose of our laboratory in the study of RNA-guided gene regulation focused on the function of small and long non-coding RNAs guiding novel nuclear events. We are performing a detailed analysis of nuclear RNA processes by studying the identity, localization, and functions of specific nuclear RNA molecules, nuclear RNA/proteins complexes and nuclear shuttling processes in Arabidopsis thaliana and Caenorhabditis elegans. Through these analyses, we expect to characterize novel nuclear RNA pathways and to uncover their biological relevance.

Selected Publications

Martín-Merchán A, Moro B, Bouet A, Bologna NG*.

Domain organization, expression, subcellular localization, and biological roles of ARGONAUTE proteins in Arabidopsis.

(2023) Journal of experimental botany vol. 74,7: 2374-2388.DOI: 10.1093/jxb/erad030.


Moro B, Kisielow M, Barragán V, Bouet A, Brosnan C, Bologna NG*.

Nuclear purification by Flow cytometry for RNA analysis.

(2021) STAR Protocols, Cell Press. DOI: 10.1016/j.xpro.2021.100320.


de Leone M.J., Hernando C.E., Romanowski A., Careno D.A., Soverna A.F., Sun H., Bologna N.G., Vázquez M., Schneeberger K., Yanovsky M.J.

Bacterial Infection Disrupts Clock Gene Expression to Attenuate Immune Responses.

(2020) Current Biology, 30(9), 1740-1747.


Rojas AML, Drusin SI, Chorostecki U, Mateos JL, Moro B, Bologna NG, Bresso EG, Schapire A, Rasia RM, Moreno DM, Palatnik JF.

Identification of key sequence features required for microRNA biogenesis in plants.

(2020) Nature Communications 11(1)5320.


Brosnan CA, Sarazin A, Lim P, Bologna NG, Hirsch-Hoffmann M, Voinnet O.

Genome-scale, single-cell-type resolution of microRNA activities within a whole plant organ.

(2019) EMBO Journal 38(13):e100754.


Bologna NG, Iselin R, Abriata L, Sarazin A, Pumplin N, Jay F, Grentzinger T, Dal Peraro M, Voinnet O.

Nucleo-cytosolic shuttling of ARGONAUTE1 prompts a revised model of the plant microRNA pathway.

(2018) Molecular Cell, 69(4), 709–719.e5.


Pumplin N, Sarazin A, Jullien PE, Bologna NG, Oberlin S, Voinnet O.

DNA Methylation Influences the Expression of DICER-LIKE4 Isoforms, Which Encode Proteins of Alternative Localization and Function. 

(2016) The Plant Cell, 28 (11) 2786-2804.


Bologna NG, Voinnet O.

The diversity, biogenesis, and activities of endogenous silencing small RNAs in Arabidopsis.

(2014) Annual Review of Plant Biology 65:473-503.


Bologna NG, Schapire AL, Zhai J, Chorostecki U, Boisbouvier J, Meyers BC, Palatnik JF.

Multiple RNA recognition patterns during microRNA biogenesis in plants.

(2013) Genome Research 23(10):1675-89.


Bologna NG, Schapire AL, Palatnik JF.

Processing of plant microRNA precursors.

(2013) Briefings in Functional Genomics 12(1):37-45.


Bologna NG, Mateos JL, Bresso EG, and Palatnik JF.

A loop-to-base processing mechanism underlies the biogenesis of plant microRNAs miR319 and miR159. 

(2009) EMBO Journal 28(23):3646-56.